CREM Co Labs has recently developed a quantitative method to assess the decontamination of high-touch environmental surfaces (HITES) by wiping and spraying in a field-relevant manner (8). The method is suitable for work with all major classes of human pathogens as well as common types of environmental surfaces. It can also assess the transfer of pathogen contamination to clean surfaces during wiping. This method, named Tier 3 of quantitative carrier test (QCT-3), builds on the two tiers (QCT-1 and QCT-2) we had developed earlier (9). QCT-3 can be used to assess the efficacy of sprays and wipes in a field-relevant manner against the surrogate of HPV (SV40).