Available Food Safety Tests

Test	Reference Method	Microbiology test
Total Microbial Count	MFHPB-33 April 2015	Enumeration of Total Aerobic Bacteria in Food Products and Food Ingredients Using 3MTM Petrifilm^TM Aerobic Count Plates
Total Microbial Count	MFHPB-18 Sep 2020 ISO 4833-1,2 :2013	Determination of the Aerobic Colony Count in foods.
Mold & Yeast Count	MFHPB-32 March 2003	Enumeration of Yeast and Mold in Food Products and Food Ingredients Using 3MTM Petrifilm^TM Yeast and Mold Count Plates
Mold & Yeast Count	MFHPB-22 Apr 2018 ISO 21527-1,2 :2008	Enumeration of Yeasts and Moulds in foods.
Coliforms	MFHPB-35 February 2001	Enumeration of Coliforms in Food Products and Food Ingredients Using 3MTM Petrifilm^TM Coliform Count Plates
	MFHPB-19 Apr 2002 ISO 4831 :2006	Detection and Enumeration of Coliforms, Faecal Coliforms and of E. coli in foods using the MPN method.
	MFHPB-31 Mar 2001	Determination of Coliforms in foods using VIOLET RED BILE AGAR
*E. coli*	MLFP-12 MLFP-30 (Box)	Detection of E. coli O157:H7 in food products by Real time PCR method
	MFHPB-34 July 2016	Enumeration of Escherichia coli and coliforms in food products and food ingredients using 3M Petrifilm^TM E. coli count plates
	MFHPB-10 Jul 2017 (former MFLP-80,90) ISO 16654:2001	Isolation of Escherichia coli O157:H7/NM from foods and environmental surface samples AMD1:2017 AMD2:2023
	MFLP-70	Detection of VTEC in food products by Real time PCR method
	ISO 7251: 2005	Detection of Escherichia coli from food and environmental samples
Salmonella spp.	MFLP-32	Detection of Salmonella spp. in food products and environmental surfaces by Real Time PCR method
Salmonella spp.	MFHPB-20 May 2023 ISO 6579-1:2017-,A1:2020	Isolation and Identification of Salmonella from food and environmental samples
*Staphylococcus aureus*	MFLP-21 July 2004	Enumeration of the Staphylococcus aureus in food and environmental samples using 3M™ Petrifilm^TM Staph express count (STX) plates
*Staphylococcus aureus*	ISO 6888-3 :2021	Detection of Staphylococcus aureus from food and environmental samples
*Campylobacter jejuni/coli/lari*	MFLP-46 Dec 2014 ISO 10272-1 :2017	Detection of Campylobacter spp. from food
*Listeria monocytogenes*	MFLP-31	Detection of Listeria monocytogenes in foods by Real Time PCR method
	MFLP-11 February 2011	Enumeration of Listeria Species in Environmental Samples using 3MJ Petrifilm J Environmental Listeria Plates
	MFHPB-30 Feb 2011, MFLP-1 Nov 2016 ISO 11290-1 :2017	Detection of Listeria monocytogenes from food and environmental samples
*Bacillus cereus*	MFLP-42 May 2011 ISO7932 :2004+A1:2020	Isolation and Enumeration of the Bacillus cereus Group in Foods
Lactic acid bacteria	Compendium 19.522	Lactic Acid Bacteria Count (Acidified MRS Agar)
Enterobacteriaceae	MFLP-09 June 2007	Enumeration of the Enterobacteriaceae species in food and environmental samples using 3M™ Petrifilm^TM Enterobacteriaceae count plates
Enterobacteriaceae	MFLP-43 ISO 21528-1,2: 2017	Determination of Enterobacteriaceae and enumeration of Enterobacteriaceae
*Shigella*	MFLP-25 Mar 2006 ISO 21567: 2004	Detection of Shigella spp. from foods (Enrichment/ culture)
*Shigella*	MFLP-26	Detection of Shigella spp. from foods by PCR
*Pseudomonas aeruginosa*	USP-62, NHP ISO 16266:2006,WATER ISO 11059, MILK ISO 13720, MEAT ISO 22717 COSMETIC	Detection and enumeration of Pseudomonas aeruginosa
Viable Spore forming microorganisms	MFLP44 Mar 2012	Determination of aerobic and anaerobic spore formers
*Yersinia enterocolitica*	MFLP-48 May 2012 ISO 10273 :2017	Isolation of Yersinia enterocolitica from foods and environmental samples
*Vibrio parahaemolyticus and/or Vibrio vulnificus*	MFLP-37 Apr 2019 ISO 21872-1	Detection, isolation, and enumeration of Vibrio parahaemolyticus and/or Vibrio vulnificus in Seafood(Enrichment/culture)
*Vibrio parahaemolyticus and/or Vibrio vulnificus*	MFLP-23	Detection, isolation, and enumeration of Vibrio parahaemolyticus by PCR method
*Clostridium perfringens*	MFHPB-23 Jun 2015 ISO 15213-2:2018	Enumeration of Clostridium perfringens in foods
*Clostridium botulinum*	MFLP-50 Jan 2012 MFHPB-16 Mar 2009 ISO 17919-2013	Detection of Clostridium botulinum in foods by Real Time PCR
*Mycobacterium tuberculosis*	Real Time PCR kit	Detection of Mycobacterium tuberculosis by Real Time PCR
*Legionella*	BS EN ISO 11731 : 2017 /PHE-FNES24	Detection and Enumeration: Legionella spp and Legionella pneumophila
*Enterococcus*	ISO 7899-1,2 : 1998 AOAC 111902	Water quality — Detection and enumeration of intestinal enterococci in surface and wastewater — Part 1: Miniaturized method (Most Probable Number) by inoculation in liquid medium
*Cyclospora cayetanensis*	OPFLP-5	Isolation and Identification of Cyclospora cayetanensis oocysts from fresh raspberries
*Giardia duodenalis*	APHA	Isolation and Identification of Giardia duodenalis– from food
Microbial Enumeration Test	USP 40 Chapter 61	Microbiological Examination of Nonsterile Products: Microbial Enumeration Test
Tests for Specified Microorganisms	USP 40 Chapter 62	Microbiological Examination of Nonsterile Products: Tests for Specified Microorganisms
Antimicrobial Effectiveness	USP 40 Chapter 51	Antimicrobial Effectiveness
Sterility test	USP 40 Chapter 71	Sterility test
Norovirus G1/G2	OPFLP-010 Mar 2010 ISO 15216-2:2019	Detection of norovirus genogroups I and II using the conventional and Real-Time Reverse- Transcriptase PCR
Hepatitis A virus	OPFLP-007 Mar 2010 ISO 15216-2:2019	Detection of Hepatitis A Virus using the conventional and Real-Time Reverse- Transcriptase PCR
Enterovirus	EPA- 1615 Sep 2014	A One-Step, Triplex, Real-Time RT-PCR Assay for the Simultaneous Detection of Enterovirus 71, Coxsackie A16 and Pan-Enterovirus in a Single Tube
Tomato Brown Rugose Virus	EPPO- PM 7/146 Number 1 Apr 2021	EPPO- PM 7/146 (1) Tomato brown rugose fruit virus
Cyclospora and Cryptosporidium	BAM chapter 19a	Detection of Cyclospora and Cryptosporidium from Fresh Produce: Isolation and Identification by Polymerase Chain Reaction (PCR) and Microscopic analysis\